6 cd49f pe conjugated antibody  (R&D Systems)

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Integrin α6-Targeted Molecular Imaging of Central Nervous System Leukemia in Mice

doi: 10.3389/fbioe.2022.812277

Figure Lengend Snippet: Surface expression of integrin α6 in human ALL cell lines and binding of the S5 peptide to NALM6 cells. (A) Flow cytometry identified the surface expression of integrin α6 in human ALL cell lines including NALM6, Ref, Jurkat, and CCRF-CEM, and (B) the highest expression of integrin α6 was observed in NALM6 cells. (C) Confocal microscopy confirmed the cellular localization of integrin α6 and the S5 peptide in NALM6 cells (Scale bar, 4 μm).

Article Snippet: NALM6, Ref, Jurkat, and CCRF-CEM cells were seeded into six-well plates, respectively, and then incubated with 10 μl/10 6 cell concentration of the anti-integrin α6 antibody (R&D, FAB13501P) and IgG2A (R&D, IC006P) at 4°C for 30 min, protected from light.

Techniques: Expressing, Binding Assay, Flow Cytometry, Confocal Microscopy

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Integrin α6-Targeted Molecular Imaging of Central Nervous System Leukemia in Mice

doi: 10.3389/fbioe.2022.812277

Figure Lengend Snippet: Pathological examination of tumor tissue. HE staining (A) identified layer-displayed leukemic cells in the superficial part of the brain parenchyma tissue, and immunohistochemical staining (B) confirmed the integrin α6 overexpression of the lesion (Scale bar, 40 μm).

Article Snippet: NALM6, Ref, Jurkat, and CCRF-CEM cells were seeded into six-well plates, respectively, and then incubated with 10 μl/10 6 cell concentration of the anti-integrin α6 antibody (R&D, FAB13501P) and IgG2A (R&D, IC006P) at 4°C for 30 min, protected from light.

Techniques: Staining, Immunohistochemical staining, Over Expression

Journal: Science Advances

Article Title: Tumor collection/processing under physioxia uncovers highly relevant signaling networks and drug sensitivity

doi: 10.1126/sciadv.abh3375

Figure Lengend Snippet: Cells for flow cytometry analysis were cultured for 5 days, and only lineage-negative (CD31-PE, CD45-PE, and CD140b-PE negative) cells were gated for the analysis. ( A ) CD49f/EpCAM staining patterns ( n = 9). ( B ) The number of CD49f + /EpCAM − cells varied on the basis of O 2 tension and tumor type. ( C ) EpCAM positivity also showed tumor-specific variability under physioxia compared to ambient air. ( D ) CD24/CD44 staining patterns of cells from ascites fluid under physioxia and ambient air ( n = 9). ( E ) Percentage of CD44 + cells in each sample under physioxia and ambient air. ( F ) Percentage of CD24 + cells under physioxia and ambient air. CD24 + cells were lower under physioxia compared to ambient air. ( G ) Cells from ascites fluids collected under physioxia contained elevated levels of BRD4 protein compared to cells collected under ambient air.

Article Snippet: The antibodies used against mouse cells were CD31-PE/Cyanine7 (A14715) from Molecular Probes; CD45-PE/Cyanine7 (25-0451-82), EpCAM-APC (allophycocyanin) (17-5791-82), and CD29-FITC (fluorescein isothiocyanate) (11-0291-82) from Invitrogen; CD140a-PE/Cyanine7 (323508) from BioLegend; LGR5-PE (phycoerythrin) (FAB8240P), TSPAN8-APC (FAB6524A), and CD49f-PE (FAB13501P) from R&D Systems; and CD61-FITC (561911), CD274-PE (558091), CD24-APC (562349), and CXCR4-FITC (551967) from BD Pharmingen; isotype control antibodies used included PE/Cyanine7 (400522) and APC (400612) from BioLegend and PE (554689) and FITC (553971) from BD Pharmingen.

Techniques: Flow Cytometry, Cell Culture, Staining